Label-free imaging and optical biomarker discovery in the tumor and tissue microenvironment with SLAM microscopy

Stephen A. Boppart

doi:10.1117/12.2607166

2 March 2022 Label-free imaging and optical biomarker discovery in the tumor and tissue microenvironment with SLAM microscopy

Stephen A. Boppart

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Proceedings Volume PC11972, Label-free Biomedical Imaging and Sensing (LBIS) 2022; PC119720E (2022) https://doi.org/10.1117/12.2607166
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Label-free intravital optical imaging, and imaging of fresh, unstained, resected tissue specimens, offer a wealth of new biomarkers for revealing the true colors of cancer and for diagnosing disease. Using new fiber-laser-pumped photonic crystal fiber source technology and nonlinear optics to generate new excitation wavelengths and manipulate the light stimulus in new ways, Simultaneous Label-free Auto-fluorescence Multi-harmonic (SLAM) microscopy can achieve fast simultaneous visualization of not only architectural features, but also the rich intrinsic molecular and metabolic features within tissues. Quantitative machine/deep learning analyses of these multi-dimensional datasets can be used to identify selective biomarkers for cancer, such as cancer-associated extracellular vesicles. The clinical demonstration of these optical biomedical imaging technologies offers new paradigms for point-of-procedure diagnosis and guidance.

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Stephen A. Boppart "Label-free imaging and optical biomarker discovery in the tumor and tissue microenvironment with SLAM microscopy", Proc. SPIE PC11972, Label-free Biomedical Imaging and Sensing (LBIS) 2022, PC119720E (2 March 2022); https://doi.org/10.1117/12.2607166

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KEYWORDS

Tissue optics

Optical imaging

Tumors

Microscopy

Biomedical optics

Cancer

Photonic crystal fibers

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