Extreme light localization for super-resolved imaging intracellular dynamics and Raman microscopy (Conference Presentation)

Donghyun Kim; Hongki Lee; Hajun Yoo; Gwiyeong Moon

doi:10.1117/12.2632604

4 October 2022 Extreme light localization for super-resolved imaging intracellular dynamics and Raman microscopy (Conference Presentation)

Donghyun Kim, Hongki Lee, Hajun Yoo, Gwiyeong Moon

Author Affiliations +

Proceedings Volume PC12203, Enhanced Spectroscopies and Nanoimaging 2022; PC122030I (2022) https://doi.org/10.1117/12.2632604
Event: SPIE Nanoscience + Engineering, 2022, San Diego, California, United States

Abstract

This work describes extreme light localization for intracellular molecular imaging and sensing with a high signal-to-noise ratio and precision. We explore localization techniques by which achievable resolution may be customized for subcellular dynamics of molecular complexes. We have also conducted plasmon-enhanced fluorescence correlation spectroscopy of cellular organelles with improved precision. The approach was extended to switching-based light localization to circumvent the diffraction limit and to use random disordered composite metallic islands for improved structured light microscopy. Extreme light localization also proves useful for enhancing Raman microscopy. Localization-based super-resolved Raman microscopy and techniques in combination with structured illumination will be discussed.

Conference Presentation

Citation Download Citation

Donghyun Kim, Hongki Lee, Hajun Yoo, and Gwiyeong Moon "Extreme light localization for super-resolved imaging intracellular dynamics and Raman microscopy (Conference Presentation)", Proc. SPIE PC12203, Enhanced Spectroscopies and Nanoimaging 2022, PC122030I (4 October 2022); https://doi.org/10.1117/12.2632604

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