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15 March 2023 Metrological flow cytometry to traceably measure the size and refractive index of single nanoparticles (Conference Presentation)
Martine Kuiper, Richard Koops, Rienk Nieuwland, Ton van Leeuwen, Edwin van der Pol
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Proceedings Volume PC12383, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI; PC1238307 (2023) https://doi.org/10.1117/12.2650545
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
Extracellular vesicles (EVs) are nanoparticles released by cells and have high potential as disease biomarkers. EVs are studied by flow cytometers, which measure scattering in arbitrary units. With Mie theory, arbitrary units can be related to diameter when the particle refractive index (RI) is known. However, a setup to determine the RI of nanoparticles in a traceable manner, including uncertainty, is lacking. Therefore, we have developed the first metrological flow cytometer, utilizing Laguerre-Gauss illumination, multi-angle light scattering, artificial intelligence data processing, and a calibrated syringe pump, to traceably determine the size, RI, and concentration of single nanoparticles in liquid.
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Martine Kuiper, Richard Koops, Rienk Nieuwland, Ton van Leeuwen, and Edwin van der Pol "Metrological flow cytometry to traceably measure the size and refractive index of single nanoparticles (Conference Presentation)", Proc. SPIE PC12383, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI, PC1238307 (15 March 2023); https://doi.org/10.1117/12.2650545
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KEYWORDS
Nanoparticles
Metrology
Particles
Refractive index
Flow cytometry
Light scattering
Optical testing
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