Differential structured illumination microscopy for 3D computational phase imaging (Conference Presentation)

Alex C. Matlock; Zahid Yaqoob; Peter T. C. So

doi:10.1117/12.2647861

15 March 2023 Differential structured illumination microscopy for 3D computational phase imaging (Conference Presentation)

Alex C. Matlock, Zahid Yaqoob, Peter T. C. So

Author Affiliations +

Proceedings Volume PC12385, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXX; PC123850F (2023) https://doi.org/10.1117/12.2647861
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

We present differential structured illumination microscopy (dSIM) for high-resolution, large field-of-view 3D computational phase imaging of biological samples. Using modified structured illuminations in a differential imaging scheme, we show our modality provides 4x resolution enhancements over the coherent imaging bandwidth while maintaining an almost 1mm2 field-of-view. This approach provides efficient reconstructions of 3D objects using a closed-form inverse scattering model. We illustrate this technique in simulation and experiment on biological samples.

Conference Presentation

Citation Download Citation

Alex C. Matlock, Zahid Yaqoob, and Peter T. C. So "Differential structured illumination microscopy for 3D computational phase imaging (Conference Presentation)", Proc. SPIE PC12385, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXX, PC123850F (15 March 2023); https://doi.org/10.1117/12.2647861

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