15 March 2023Label-free visualization of chromatin remodeling in live cell nuclei by analyzing the dynamic interference scattering signal (Conference Presentation)
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We present a novel optical microscope technique to detect the chromatin organization and DNA damage foci in the unlabeled live cell nuclei. The dynamic scattering signal of chromatin is recorded by a highly sensitive interference microscopy, contrast-enhanced coherent brightfield microscopy (COBRI), at a high speed. By analyzing the temporal fluctuations of the scattering signal, the density and the compaction level of chromatin are accurately estimated with the sub-micrometer spatial resolutions. This imaging strategy is referred to as “DYNAMICS imaging”. Using DYNAMICS imaging, we monitor the dynamic chromatin remodeling initiated by DNA damage.
Yi-Teng Hsiao,Fasih Bintang Ilhami,I-Hsin Liao, andChia-Lung Hsieh
"Label-free visualization of chromatin remodeling in live cell nuclei by analyzing the dynamic interference scattering signal (Conference Presentation)", Proc. SPIE PC12385, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXX, PC123850L (15 March 2023); https://doi.org/10.1117/12.2646966
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Yi-Teng Hsiao, Fasih Bintang Ilhami, I-Hsin Liao, Chia-Lung Hsieh, "Label-free visualization of chromatin remodeling in live cell nuclei by analyzing the dynamic interference scattering signal (Conference Presentation)," Proc. SPIE PC12385, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXX, PC123850L (15 March 2023); https://doi.org/10.1117/12.2646966