Imaging prostate tumor spheroids with dynamic contrast line-field optical coherence tomography (LF-dOCT)

Stephanie Swanson; Keyu Chen; Saduni U. Sumithrarachchige Don; Ernest Osei; Kostadinka Bizheva

doi:10.1117/12.3005692

13 March 2024 Imaging prostate tumor spheroids with dynamic contrast line-field optical coherence tomography (LF-dOCT)

Stephanie Swanson, Keyu Chen, Saduni U. Sumithrarachchige Don, Ernest Osei, Kostadinka Bizheva

Author Affiliations +

Proceedings Volume PC12830, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVIII; PC128302K (2024) https://doi.org/10.1117/12.3005692
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Prostate cancer is the most prevalent cancer among Canadian men. Historically, cancer has been studied using 2D cell culture methods, but recently 3D cell culture methods like tumor spheroids have been become popular to better replicate the physiological environment of the body. We developed ultrahigh resolution line-field OCT (LF-OCT) technology and a dynamic OCT (dOCT) method to image tumor spheroids and identify areas of necrosis, for comparison with computer simulation. The LF-OCT system has sufficient spatial resolution to identify individual cells and capture the cellular level of activity while the dOCT algorithm can resolve the dynamic activity accurately. The study will be extended to investigate the spatial and temporal development of necrosis in PC3 prostate tumor spheroids under different conditions like such as temperature, pressure, and culture medium type. These results will be used to inform and extend the model to an in vivo environment.

Conference Presentation

Citation Download Citation

Stephanie Swanson, Keyu Chen, Saduni U. Sumithrarachchige Don, Ernest Osei, and Kostadinka Bizheva "Imaging prostate tumor spheroids with dynamic contrast line-field optical coherence tomography (LF-dOCT)", Proc. SPIE PC12830, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVIII, PC128302K (13 March 2024); https://doi.org/10.1117/12.3005692

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