Presentation
13 March 2024 Using meditope-based antibody labeling to improve fluorescence lifetime FRET imaging
Amit Verma, Catherine Sherry, Nanxue Yuan, Vikas Pandey, John Williams, Xavier Intes, Margarida M. Barroso
Author Affiliations +
Abstract
Herein, we will use Macroscopy Fluorescence Lifetime Forster Resonance Energy Transfer (MFLI FRET) to quantitatively image in vivo and non-invasively the efficacy of receptor-targeted drug delivery systems. We have established an antibody-to-target imaging pipeline to develop novel near-infrared (NIR)-labeled probes to interrogate and quantify the dynamics of drug-receptor binding in HER2 breast cancer tumor xenografts.
Conference Presentation
© (2024) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Amit Verma, Catherine Sherry, Nanxue Yuan, Vikas Pandey, John Williams, Xavier Intes, and Margarida M. Barroso "Using meditope-based antibody labeling to improve fluorescence lifetime FRET imaging", Proc. SPIE PC12847, Multiphoton Microscopy in the Biomedical Sciences XXIV, PC128470S (13 March 2024); https://doi.org/10.1117/12.3008753
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KEYWORDS
Antibodies

Fluorescence resonance energy transfer

Biological imaging

Fluorescence

Near infrared

Tumors

Fluorescence imaging

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