Preservation media analysis for ex vivo measurements of endogenous UV fluorescence of liver fibrosis in bulk samples

Enoch Gutierrez-Herrera; Adolfo Perez-Garcia D.V.M.; Nathalie Aleman-García; Antonio Ortega-Martinez; Celia Sánchez-Pérez; Walfre Franco; Joselín Hernández-Ruiz

doi:10.1117/12.2252795

15 February 2017 Preservation media analysis for ex vivo measurements of endogenous UV fluorescence of liver fibrosis in bulk samples

Enoch Gutierrez-Herrera, Adolfo Perez-Garcia D.V.M., Nathalie Aleman-García, Antonio Ortega-Martinez, Celia Sánchez-Pérez, Walfre Franco, Joselín Hernández-Ruiz

Author Affiliations +

Proceedings Volume 10062, Optical Interactions with Tissue and Cells XXVIII; 100621C (2017) https://doi.org/10.1117/12.2252795
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Non-subjective, minimally-invasive, and quantifying techniques may support development and evaluation of a fibrosis regression treatment. The build-up of extracellular matrix in liver fibrosis may result on changes of the endogenous fluorescence of tissue. In this work, we evaluate the fluorescence excitation/emission matrix in the UV range for several bulk samples of murine hepatic tissue preserved in different media. Chemical changes on tissue, caused by formaldehyde preservation, alter the endogenous fluorescence spectra. To avoid these drawbacks, phosphate-buffered saline (PBS) or Iscove’s Modified Dulbecco’s Medium were used. PBS buffer showed to be the less harmful and cost-effective preservation medium to study the endogenous fluorescence in fibrotic tissue.

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Enoch Gutierrez-Herrera, Adolfo Perez-Garcia D.V.M., Nathalie Aleman-García, Antonio Ortega-Martinez, Celia Sánchez-Pérez, Walfre Franco, and Joselín Hernández-Ruiz "Preservation media analysis for ex vivo measurements of endogenous UV fluorescence of liver fibrosis in bulk samples", Proc. SPIE 10062, Optical Interactions with Tissue and Cells XXVIII, 100621C (15 February 2017); https://doi.org/10.1117/12.2252795

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