Identification of intramural metastasis in esophageal cancer using multiphoton microscopy

Jian Xu; Deyong Kang; Shuangmu Zhuo; Xiaoqin Zhu; jiangbo Lin; Jianxin Chen

doi:10.1117/12.2250766

21 February 2017 Identification of intramural metastasis in esophageal cancer using multiphoton microscopy

Jian Xu, Deyong Kang, Shuangmu Zhuo, Xiaoqin Zhu, jiangbo Lin, Jianxin Chen

Proceedings Volume 10069, Multiphoton Microscopy in the Biomedical Sciences XVII; 100691R (2017) https://doi.org/10.1117/12.2250766
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Intramural metastasis (IM) of esophageal cancer is defined as metastasis from a primary lesion to the esophageal wall without intraepithelial cancer extension. Esophageal cancer with IM is more common and such cases indicate a poor prognosis. In esophageal surgery, if curative resection is possible, the complete removal of both primary tumor and associated IMs is required. Therefore, accurate diagnosis of IMs in esophageal cancer prior to surgery is of particular importance. Multiphoton microscopy (MPM) with subcellular resolution is well-suited for deep tissue imaging since many endogenous fluorophores of fresh biological tissues are excited through two-photon excited fluorescence (TPEF) and second harmonic generation (SHG). Here, a study to identify IM in fresh tissue section using MPM is reported. In this study, the morphological and spectral differences between IM and surrounding tissue are described. These results show that MPM has the ability to accurately identify IM in esophageal tissues. With improvement of the penetration depth of MPM and the development of multiphton microendoscope, MPM may be a promising imaging technique for preoperative diagnosis of IMs in esophageal cancer in the future.

Citation Download Citation

Jian Xu, Deyong Kang, Shuangmu Zhuo, Xiaoqin Zhu, jiangbo Lin, and Jianxin Chen "Identification of intramural metastasis in esophageal cancer using multiphoton microscopy", Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 100691R (21 February 2017); https://doi.org/10.1117/12.2250766

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available