Three-dimensional fluorescence imaging of particles in a glass capillary using tilted light-sheet illumination

Masaya Okada; Shigeki Iwanaga

doi:10.1117/12.2253540

21 September 2017 Three-dimensional fluorescence imaging of particles in a glass capillary using tilted light-sheet illumination

Masaya Okada, Shigeki Iwanaga

Author Affiliations +

Proceedings Volume 10076, High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management II; 1007616 (2017) https://doi.org/10.1117/12.2253540
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

We have attempted to image three-dimensional distribution of fluorescence beads embedded within gels in a glass capillary using light-sheet illumination, as a primary step towards developing a three-dimensional molecular imaging flow cytometer. An illumination and a detection path were arranged orthogonal to the longer axis of the capillary. The light-sheet illumination was tilted with respect to the illumination axis to image a projection of a section of the sample by a CCD. Different sections of the sample were imaged through scanning the capillary itself, along its length. By stacking the images after inverse transformation, the three-dimensional distribution of the fluorescence beads was imaged.

Citation Download Citation

Masaya Okada and Shigeki Iwanaga "Three-dimensional fluorescence imaging of particles in a glass capillary using tilted light-sheet illumination", Proc. SPIE 10076, High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management II, 1007616 (21 September 2017); https://doi.org/10.1117/12.2253540

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