Investigation of light scattering characteristics of individual leukocytes using three-dimensional refractive index maps

Kung-Bin Sung; Yang-Hsien Lin; Fong-jheng Lin; Chao-Mao Hsieh; Shang-Ju Wu

doi:10.1117/12.2270427

18 April 2017 Investigation of light scattering characteristics of individual leukocytes using three-dimensional refractive index maps

Kung-Bin Sung, Yang-Hsien Lin, Fong-jheng Lin, Chao-Mao Hsieh, Shang-Ju Wu

Author Affiliations +

Proceedings Volume 10251, Biomedical Imaging and Sensing Conference; 1025109 (2017) https://doi.org/10.1117/12.2270427
Event: SPIE Technologies and Applications of Structured Light, 2017, Yokohama, Japan

Abstract

Three-dimensional (3D) refractive-index (RI) microscopy is an emerging technique suitable for live-cell imaging due to its label-free and fast 3D imaging capabilities. We have developed a common-path system to acquire 3D RI microscopic images of cells with excellent speed and stability. After obtaining 3D RI distributions of individual leukocytes, we used a 3D finite-difference time-domain tool to study light scattering properties. Backscattering spectra of lymphocytes, monocytes and neutrophils are different from each other. Backscattering spectra of lymphocytes matched well with those of homogeneous spheres as predicted by Mie theory while backscattering spectra of neutrophils are significantly more intense than those of the other two types. This suggests the possibility of classifying the three types of leukocytes based on backscattering.

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Kung-Bin Sung, Yang-Hsien Lin, Fong-jheng Lin, Chao-Mao Hsieh, and Shang-Ju Wu "Investigation of light scattering characteristics of individual leukocytes using three-dimensional refractive index maps", Proc. SPIE 10251, Biomedical Imaging and Sensing Conference, 1025109 (18 April 2017); https://doi.org/10.1117/12.2270427

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KEYWORDS

Backscatter

Light scattering

3D image processing

Finite-difference time-domain method

Microscopy

Refractive index

Diffraction

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