Assessment of imaging quality in magnified phase CT of human bone tissue at the nanoscale

Boliang Yu; Max Langer; Alexandra Pacureanu; Remy Gauthier; Helene Follet; David Mitton; Cecile Olivier; Peter Cloetens; Francoise Peyrin

doi:10.1117/12.2272561

3 October 2017 Assessment of imaging quality in magnified phase CT of human bone tissue at the nanoscale

Boliang Yu, Max Langer, Alexandra Pacureanu, Remy Gauthier, Helene Follet, David Mitton, Cecile Olivier, Peter Cloetens, Francoise Peyrin

Author Affiliations +

Proceedings Volume 10391, Developments in X-Ray Tomography XI; 103910L (2017) https://doi.org/10.1117/12.2272561
Event: SPIE Optical Engineering + Applications, 2017, San Diego, California, United States

Abstract

Bone properties at all length scales have a major impact on the fracture risk in disease such as osteoporosis. However, quantitative 3D data on bone tissue at the cellular scale are still rare. Here we propose to use magnified X-ray phase nano-CT to quantify bone ultra-structure in human bone, on the new setup developed on the beamline ID16A at the ESRF, Grenoble. Obtaining 3D images requires the application of phase retrieval prior to tomographic reconstruction. Phase retrieval is an ill-posed problem for which various approaches have been developed. Since image quality has a strong impact on the further quantification of bone tissue, our aim here is to evaluate different phase retrieval methods for imaging bone samples at the cellular scale. Samples from femurs of female donors were scanned using magnified phase nano-CT at voxel sizes of 120 and 30 nm with an energy of 33 keV. Four CT scans at varying sample-to-detector distances were acquired for each sample. We evaluated three phase retrieval methods adapted to these conditions: Paganin’s method at single distance, Paganin’s method extended to multiple distances, and the contrast transfer function (CTF) approach for pure phase objects. These methods were used as initialization to an iterative refinement step. Our results based on visual and quantitative assessment show that the use of several distances (as opposed to single one) clearly improves image quality and the two multi-distance phase retrieval methods give similar results. First results on the segmentation of osteocyte lacunae and canaliculi from such images are presented.

Conference Presentation

Citation Download Citation

Boliang Yu, Max Langer, Alexandra Pacureanu, Remy Gauthier, Helene Follet, David Mitton, Cecile Olivier, Peter Cloetens, and Francoise Peyrin "Assessment of imaging quality in magnified phase CT of human bone tissue at the nanoscale", Proc. SPIE 10391, Developments in X-Ray Tomography XI, 103910L (3 October 2017); https://doi.org/10.1117/12.2272561

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available