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8 February 2018 Use of a conformational switching aptamer for rapid and specific ex vivo identification of central nervous system lymphoma in a xenograft model
Joseph F. Georges, Xiaowei Liu, Jennifer Eschbacher, Joshua Nichols, Michael A. Mooney, Anna Joy, Robert F. Spetzler, Burt G. Feuerstein , Trent Anderson, Mark C. Preul, Hao Yan, Peter Nakaji
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Proceedings Volume 10480, Clinical and Translational Neurophotonics 2018; 104800G (2018) https://doi.org/10.1117/12.2290883
Event: SPIE BiOS, 2018, San Francisco, California, United States
Abstract
Improved tools for providing specific intraoperative diagnoses could improve patient care. In neurosurgery, intraoperatively differentiating non-operative lesions can be challenging, often necessitating immunohistochemical (IHC) procedures which require up to 24-48 hours. Here, we evaluate the feasibility of generating rapid ex vivo specific labeling using a novel lymphoma-specific fluorescent switchable aptamer. Our B-cell lymphoma-specific switchable aptamer produced only low-level fluorescence in its unbound conformation and generated an 8-fold increase in fluorescence once bound to its target on CD20-positive lymphoma cells. The aptamer demonstrated strong binding to B-cell lymphoma cells within 10 minutes of incubation. We applied the switchable aptamer to ex vivo xenograft tissue harboring B-cell lymphoma and astrocytoma, and within one hour specific visual identification of lymphoma was routinely possible. In this proof-of-concept study in human cell culture and orthotopic xenografts, we conclude that a fluorescent switchable aptamer can provide rapid and specific labeling of B-cell lymphoma, and that developing aptamer-based labeling approaches could simplify tissue staining and drastically reduce time to histopathological diagnoses compared with IHC-based methods. We propose that switchable aptamers could enhance expeditious, accurate intraoperative decision-making.
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Joseph F. Georges, Xiaowei Liu, Jennifer Eschbacher, Joshua Nichols, Michael A. Mooney, Anna Joy, Robert F. Spetzler, Burt G. Feuerstein , Trent Anderson, Mark C. Preul, Hao Yan, and Peter Nakaji "Use of a conformational switching aptamer for rapid and specific ex vivo identification of central nervous system lymphoma in a xenograft model", Proc. SPIE 10480, Clinical and Translational Neurophotonics 2018, 104800G (8 February 2018); https://doi.org/10.1117/12.2290883
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KEYWORDS
Lymphoma
Tissues
Luminescence
Diagnostics
Tumors
Brain
Nervous system
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