Quantifying cancer cell receptors with paired-agent fluorescent imaging: a novel method to account for tissue optical property effects

Negar Sadeghipour; Scott C. Davis; Kenneth M. Tichauer

doi:10.1117/12.2290631

20 February 2018 Quantifying cancer cell receptors with paired-agent fluorescent imaging: a novel method to account for tissue optical property effects

Negar Sadeghipour, Scott C. Davis, Kenneth M. Tichauer

Author Affiliations +

Proceedings Volume 10497, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI; 1049723 (2018) https://doi.org/10.1117/12.2290631
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Dynamic fluorescence imaging approaches can be used to estimate the concentration of cell surface receptors in vivo. Kinetic models are used to generate the final estimation by taking the targeted imaging agent concentration as a function of time. However, tissue absorption and scattering properties cause the final readout signal to be on a different scale than the real fluorescent agent concentration. In paired-agent imaging approaches, simultaneous injection of a suitable control imaging agent with a targeted one can account for non-specific uptake and retention of the targeted agent. Additionally, the signal from the control agent can be a normalizing factor to correct for tissue optical property differences. In this study, the kinetic model used for paired-agent imaging analysis (i.e., simplified reference tissue model) is modified and tested in simulation and experimental data in a way that accounts for the scaling correction within the kinetic model fit to the data to ultimately extract an estimate of the targeted biomarker concentration.

Citation Download Citation

Negar Sadeghipour, Scott C. Davis, and Kenneth M. Tichauer "Quantifying cancer cell receptors with paired-agent fluorescent imaging: a novel method to account for tissue optical property effects", Proc. SPIE 10497, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI, 1049723 (20 February 2018); https://doi.org/10.1117/12.2290631

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available