Enhancement of measurement speed and photon economy in multiphoton detected fluorescence lifetime imaging microscopy

Dongeun Kim; Wonsang Hwang; Youngjae Won; Sucbei Moon; Dug Young Kim

doi:10.1117/12.2290059

23 February 2018 Enhancement of measurement speed and photon economy in multiphoton detected fluorescence lifetime imaging microscopy

Dongeun Kim, Wonsang Hwang, Youngjae Won, Sucbei Moon, Dug Young Kim

Author Affiliations +

Proceedings Volume 10498, Multiphoton Microscopy in the Biomedical Sciences XVIII; 104982G (2018) https://doi.org/10.1117/12.2290059
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

The Analog mean delay (AMD) method is a multiphoton detection fluorescence lifetime measurement method. We have investigated the effect of the linearity of a photodetector response on the performance of the AMD method, which is a multiphoton detected fluorescence lifetime measurement method. A Monte-Carlo simulation scheme was adapted to generate various electronic signals for the AMD method with different linearity conditions of a photodetector. It is found that the photon economy is better if a photodetector with better linearity is used especially when the number of detected photons per pulse is low.

Citation Download Citation

Dongeun Kim, Wonsang Hwang, Youngjae Won, Sucbei Moon, and Dug Young Kim "Enhancement of measurement speed and photon economy in multiphoton detected fluorescence lifetime imaging microscopy", Proc. SPIE 10498, Multiphoton Microscopy in the Biomedical Sciences XVIII, 104982G (23 February 2018); https://doi.org/10.1117/12.2290059

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