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20 February 2018 Video-rate confocal phase imaging by use of scan-less dual comb microscopy
Takahiko Mizuno, Eiji Hase, Takeo Minamikawa, Hirotsugu Yamamoto, Takeshi Yasui
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Proceedings Volume 10505, High-Speed Biomedical Imaging and Spectroscopy III: Toward Big Data Instrumentation and Management; 1050508 (2018) https://doi.org/10.1117/12.2289231
Event: SPIE BiOS, 2018, San Francisco, California, United States
Abstract
We demonstrate the confocal phase imaging at a video rate by a combination of dual comb spectroscopy (DSC) with 2D spectral encoding (2D-SE). After the image pixels of the sample is encoded on an optical frequency comb (OFC) by 2DSE, DSC of the image-encoded OFC passing through the confocal pinhole gives the mode-resolved amplitude and phase spectra. Based on one-to-one correspondence between the image pixels and OFC modes, the confocal amplitude and phase images are decoded from the mode-resolved amplitude and phase spectra, respectively. The phase spectrum measurement without the need for mechanical scanning enables the video-rate confocal phase imaging.
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Takahiko Mizuno, Eiji Hase, Takeo Minamikawa, Hirotsugu Yamamoto, and Takeshi Yasui "Video-rate confocal phase imaging by use of scan-less dual comb microscopy", Proc. SPIE 10505, High-Speed Biomedical Imaging and Spectroscopy III: Toward Big Data Instrumentation and Management, 1050508 (20 February 2018); https://doi.org/10.1117/12.2289231
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KEYWORDS
Confocal microscopy
Phase imaging
Microscopy
Image resolution
Optical microscopy
Spectroscopy
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