Incoherent holographic imaging of subsurface structures with volume holographic gratings

Yu-Hsin Chia; Hung-Chun Wang; Yuan Luo

doi:10.1117/12.2319365

24 April 2018 Incoherent holographic imaging of subsurface structures with volume holographic gratings

Yu-Hsin Chia, Hung-Chun Wang, Yuan Luo

Proceedings Volume 10711, Biomedical Imaging and Sensing Conference; 107111A (2018) https://doi.org/10.1117/12.2319365
Event: SPIE Structured Light, 2018, Yokohama, Japan

Abstract

Under broadband illumination, a multiplane microscopy incorporating volume holographic gratings (VHGs) to observe three-dimensional structures of biological samples with different depth simultaneously is presented. VHGs formed in thick recording materials, including PQ-PMMA, provide strong angular and wavelength transmittance filtering properties, which enable acquiring spatial–spectral images of fine structures within biological samples. Here, we experimentally demonstrate this microscopic imaging capability to obtain multiple depth-resolved mixed pollen grains images of fine structures from eight depths in one shot.

Citation Download Citation

Yu-Hsin Chia, Hung-Chun Wang, and Yuan Luo "Incoherent holographic imaging of subsurface structures with volume holographic gratings", Proc. SPIE 10711, Biomedical Imaging and Sensing Conference, 107111A (24 April 2018); https://doi.org/10.1117/12.2319365

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