Determination of aloin in aloe extracts from different companies by HPLC-UV

Xiang-Ping Meng; Zi-Cong Wu; Fan Shi; Yi-Fei Wang; Zhi-ping Wang; Tong-sheng Chen

doi:10.1117/12.2500150

23 October 2018 Determination of aloin in aloe extracts from different companies by HPLC-UV

Xiang-Ping Meng, Zi-Cong Wu, Fan Shi, Yi-Fei Wang, Zhi-ping Wang, Tong-sheng Chen

Proceedings Volume 10820, Optics in Health Care and Biomedical Optics VIII; 108201B (2018) https://doi.org/10.1117/12.2500150
Event: SPIE/COS Photonics Asia, 2018, Beijing, China

Abstract

To give some foundation for quality standards of the aloe extracts, by studying the contents of the aloin in aloe extracts and evaluating scientifically the quality of the aloe extracts from different companies. The chromatographic separation was performed on a SunFire C₁₈ column (250mm×4.6mm, 5 μm). The mobile phase comprised acetonitrile-water (25:75, V/V,) at a flow rate of 1.0 mL/min. The detection wavelength was set at 355nm. There was a great difference in the aloe extracts from different companies. There was a good linear relationship between peak area and the injection volume over the range of 2.5-20μL (R² = 0.9992). The average spike recovery of the aloin was 99.62%, With a RSD of 1.20%(n=6). The contents of aloe extracts from different companies were 90.64-263.11mg/g. This method is simple, fast and the result is accurate and reliable and can be used for the determination of the aloin in aloe extracts.

Citation Download Citation

Xiang-Ping Meng, Zi-Cong Wu, Fan Shi, Yi-Fei Wang, Zhi-ping Wang, and Tong-sheng Chen "Determination of aloin in aloe extracts from different companies by HPLC-UV", Proc. SPIE 10820, Optics in Health Care and Biomedical Optics VIII, 108201B (23 October 2018); https://doi.org/10.1117/12.2500150

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