Single cell detection using multimodal photoacoustic microscopy, optical coherence tomography, and fluorescence microscopy (Conference Presentation)

Van Phuc Nguyen; Yannis M. Paulus; Xueding Wang; Wei Qian; Yanxiu Li; Wei Zhang

doi:10.1117/12.2510781

4 March 2019 Single cell detection using multimodal photoacoustic microscopy, optical coherence tomography, and fluorescence microscopy (Conference Presentation)

Van Phuc Nguyen, Yannis M. Paulus, Xueding Wang, Wei Qian, Yanxiu Li, Wei Zhang

Author Affiliations +

Proceedings Volume 10881, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVII; 1088111 (2019) https://doi.org/10.1117/12.2510781
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Successful development of a multimodal imaging system for the detection of live single cells is critical for enhancing diagnosis and treatment of diseases at an early stage. Recently, photoacoustic microscopy (PAM) and optical coherence tomography (OCT) have been investigated as high resolution, non-invasive imaging techniques for the detection of single cells. However, most cancerous cells lack sufficient endogenous contrast. The aim of the current study is to develop a multimodal imaging system that combines PAM, OCT, and fluorescence microscopy (FM) for the detection of single cells with the assistance of gold nanoparticles as contrast agents. Three different type of ultra-pure colloidal cluster gold nanoparticles (GNPs) were fabricated and functionalized with ligands. The synthesized GNPs, average particle size of 10, 20 nm, and 60 nm (cluster GNPs), were evaluated. Cluster nanoparticles were achieved by combining an average of 3 to4 colloidal GNPs. The peak absorption of GNPs is 532 nm (bare) and 650 nm (cluster). The functionalized GNPs were delivered into different cells (HeLa, OZ3, bovine retinal endothelial, and bovine brain endothelial cells) at various concentrations (0, 12.5, 25 and 50 µg/mL). The treated cells with GNPs were imaged using the custom-built multimodal PAM, OCT, and FM system. The PAM contrast was enhanced linearly with the increasing in GNP concentration. The OCT contrast was also increased up to 2 fold in comparison with control. FM results showed the position of accumulated GNPs co-registered with the dark field images. The proposed multimodal imaging system may provide a potential tool for the detection and imaging of live single cells.

Conference Presentation

Citation Download Citation

Van Phuc Nguyen, Yannis M. Paulus, Xueding Wang, Wei Qian, Yanxiu Li, and Wei Zhang "Single cell detection using multimodal photoacoustic microscopy, optical coherence tomography, and fluorescence microscopy (Conference Presentation)", Proc. SPIE 10881, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVII, 1088111 (4 March 2019); https://doi.org/10.1117/12.2510781

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KEYWORDS

Optical coherence tomography

Luminescence

Microscopy

Photoacoustic microscopy

Imaging systems

Fermium

Frequency modulation

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