Feasibility study of limited-angle reconstruction based in vivo optical projection tomography

Nan Wang; Duofang Chen; Cuiping Bao; Jimin Liang; Xueli Chen; Shouping Zhu

doi:10.1117/12.2509485

21 February 2019 Feasibility study of limited-angle reconstruction based in vivo optical projection tomography

Nan Wang, Duofang Chen, Cuiping Bao, Jimin Liang, Xueli Chen, Shouping Zhu

Proceedings Volume 10883, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVI; 108830S (2019) https://doi.org/10.1117/12.2509485
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Optical projection tomography(OPT) provides an approach to recreating three-dimensional images of small biological specimens. Light traverses through a straight line to achieve a homogeneous illumination of the specimen. As the specimens in the conventional OPT could not survive or the survival time was too short, this paper proposes a new type of sample fixation method for OPT imaging. The specimen was anaesthetized in a petri dish, and the dish was fixed under the rotational stage of our homemade OPT system for imaging. This method can reduce the damage to the specimen and be more conducive to the continuous observation for in vivo OPT. However, the sample fixation causes the problem of insufficient sampling. To obtain optical projection tomographic image with insufficient samples, this paper uses the iterative reconstruction algorithm combining with the prior information to solve the inverse reconstruction problem.

Conference Presentation

Citation Download Citation

Nan Wang, Duofang Chen, Cuiping Bao, Jimin Liang, Xueli Chen, and Shouping Zhu "Feasibility study of limited-angle reconstruction based in vivo optical projection tomography", Proc. SPIE 10883, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVI, 108830S (21 February 2019); https://doi.org/10.1117/12.2509485

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