Layer-by-layer nano-assembly of charged polyelectrolytes for label-free optical biosensing with nanostructured materials: the case of nanostructured porous silicon interferometers

S. Mariani; V. Robbiano; L. M. Strambini; A. Debrassi; G. Egri; L. Dähne; G. Barillaro

doi:10.1117/12.2508847

7 March 2019 Layer-by-layer nano-assembly of charged polyelectrolytes for label-free optical biosensing with nanostructured materials: the case of nanostructured porous silicon interferometers

S. Mariani, V. Robbiano, L. M. Strambini, A. Debrassi, G. Egri, L. Dähne, G. Barillaro

Author Affiliations +

Proceedings Volume 10895, Frontiers in Biological Detection: From Nanosensors to Systems XI; 1089503 (2019) https://doi.org/10.1117/12.2508847
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

The nano-assembly of charged polyelectrolytes via layer-by-layer (LbL) technology on porous silicon (PSi) interferometers is here demonstrated as an effective biofunctionalization approach for high-sensitivity/selectivity labelfree optical biosensing, using streptavidin/biotin affinity detection as case study. Nanostructured PSi interferometers are biofunctionalized with a nano-assembly of a positively-charged polyelectrolyte, namely, PAH (poly(allylamine hydrochloride)), and a negatively-charged biotinylated polyelectrolyte, namely, b-PMAA (poly(methacrylic acid)), via LbL technology. The nano-assembly is stable under operating conditions and enables the selective and sensitive detection of streptavidin with a sub-picomolar detection limit (namely, DL=0.6 pM), which is 10⁵-fold lower than that achieved with PSi interferometers biofunctionalized using standard silane chemistry. Remarkably, the analytical performance achieved for LbL-biofunctionalized PSi interferometers is comparable to those of state-of-the-art label-free photonic and plasmonic platforms.

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Citation Download Citation

S. Mariani, V. Robbiano, L. M. Strambini, A. Debrassi, G. Egri, L. Dähne, and G. Barillaro "Layer-by-layer nano-assembly of charged polyelectrolytes for label-free optical biosensing with nanostructured materials: the case of nanostructured porous silicon interferometers", Proc. SPIE 10895, Frontiers in Biological Detection: From Nanosensors to Systems XI, 1089503 (7 March 2019); https://doi.org/10.1117/12.2508847

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