Imaging biomarkers quantify therapeutic effect in 3D-printed skin cancer constructs (Conference Presentation)

Daniel S. Gareau; James Browning; Marc Ferrer; John A. Carucci; Samantha R. Lish

doi:10.1117/12.2550832

9 March 2020 Imaging biomarkers quantify therapeutic effect in 3D-printed skin cancer constructs (Conference Presentation)

Daniel S. Gareau, James Browning, Marc Ferrer, John A. Carucci, Samantha R. Lish

Author Affiliations +

Proceedings Volume 11243, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII; 112431G (2020) https://doi.org/10.1117/12.2550832
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Chemical space for small molecule therapeutics discovery is greatly under-explored due to difficulties in animal testing, the first bottleneck compounds encounter in going from formula to human use. We developed and validated an assay that combines 3D tissue biofabrication with high-throughput imaging biomarkers. This may impact more diseases than just skin cancer, where we have recently shown promising preliminary findings. Our skin constructs have normal epidermis, with populations of human keratinocytes, dermis with human fibroblasts and tumor spheroids containing populations of human squamous cell carcinoma cells. We present imaging biomarkers that show the cellular chemotherapeutic treatment. This constitutes a novel chemotherapeutic assay that may enable a paradigm-shifting drug discovery pipeline. Such a pipeline could enable tissue-relevant assay on a high throughput scale and be both more robust than monolayer cell culture and easier than animal models.

Conference Presentation

Citation Download Citation

Daniel S. Gareau, James Browning, Marc Ferrer, John A. Carucci, and Samantha R. Lish "Imaging biomarkers quantify therapeutic effect in 3D-printed skin cancer constructs (Conference Presentation)", Proc. SPIE 11243, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII, 112431G (9 March 2020); https://doi.org/10.1117/12.2550832

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KEYWORDS

Skin cancer

Tissues

Animal testing

Tumors

3D image processing

Cancer

Confocal microscopy

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