Scanning FCS and super-resolution microscopy on 2D lipid membranes (Conference Presentation)

Uwe Ortmann; Mariano Gonzalez Pisfil; Marcelle König; Rhys Dowler; Benedikt Krämer; Sumeet Rohilla; Felix Koberling; Rainer Erdmann

doi:10.1117/12.2542417

26 March 2020 Scanning FCS and super-resolution microscopy on 2D lipid membranes (Conference Presentation)

Uwe Ortmann, Mariano Gonzalez Pisfil, Marcelle König, Rhys Dowler, Benedikt Krämer, Sumeet Rohilla, Felix Koberling, Rainer Erdmann

Author Affiliations +

Proceedings Volume 11246, Single Molecule Spectroscopy and Superresolution Imaging XIII; 1124607 (2020) https://doi.org/10.1117/12.2542417
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Scanning FCS (sFCS) is a great tool for studying slowly diffusing species as is often the case in cell membranes. In sFCS, the excitation volume is scanned rapidly through the sample allowing for simultaneous measurement at multiple locations. The shorter residence times also lead to lower photon doses experienced by each detected molecule, reducing the risk of photobleaching. Here, we show results from sFCS measurements on supported lipid bilayers (SLBs) where fluorescence lifetime information is used to achieve an axial nanometric localization based on Metal Induced Energy Transfer (MIET).

Conference Presentation

Citation Download Citation

Uwe Ortmann, Mariano Gonzalez Pisfil, Marcelle König, Rhys Dowler, Benedikt Krämer, Sumeet Rohilla, Felix Koberling, and Rainer Erdmann "Scanning FCS and super-resolution microscopy on 2D lipid membranes (Conference Presentation)", Proc. SPIE 11246, Single Molecule Spectroscopy and Superresolution Imaging XIII, 1124607 (26 March 2020); https://doi.org/10.1117/12.2542417

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