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10 March 2020 Fingerprint–to-CH stretch region high spectral resolution Stimulated Raman Scattering microscopy with an Acousto Optical Tunable Filter (Conference Presentation)
Sergey P. Laptenok, Luca Genchi, Vijayakumar P. Rajamanickam, Andrea Bertoncini, Alessandro Giammona, Carlo Liberale
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Proceedings Volume 11251, Label-free Biomedical Imaging and Sensing (LBIS) 2020; 112511B (2020) https://doi.org/10.1117/12.2543972
Event: SPIE BiOS, 2020, San Francisco, California, United States
Abstract
We present a novel design for a Stimulated Raman Scattering (SRS) microscope based on a dual beam femtosecond laser in combination with spectral shaping through a fast and narrowband Acousto Optical Tunable Filter. This configuration allows the measurement of broad SRS spectra, all the way from fingerprint region to CH stretch region without any modification of the optical setup. High spectral resolution over a broad spectral region allows label-free quantitative imaging of biological samples. We will show the application of our SRS system to a quantitative study of lipid droplets in colon Cancer Stem Cells.
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Sergey P. Laptenok, Luca Genchi, Vijayakumar P. Rajamanickam, Andrea Bertoncini, Alessandro Giammona, and Carlo Liberale "Fingerprint–to-CH stretch region high spectral resolution Stimulated Raman Scattering microscopy with an Acousto Optical Tunable Filter (Conference Presentation)", Proc. SPIE 11251, Label-free Biomedical Imaging and Sensing (LBIS) 2020, 112511B (10 March 2020); https://doi.org/10.1117/12.2543972
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KEYWORDS
Microscopy
Raman scattering
Spectral resolution
Tunable filters
Microscopes
Raman spectroscopy
Femtosecond phenomena
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