Full-field interferometric imaging of neuron deformation during action potential (Conference Presentation)

Tong Ling; Kevin C. Boyle; Valentina Zuckerman; Thomas Flores; Daniel V. Palanker

doi:10.1117/12.2543625

10 March 2020 Full-field interferometric imaging of neuron deformation during action potential (Conference Presentation)

Tong Ling, Kevin C. Boyle, Valentina Zuckerman, Thomas Flores, Daniel V. Palanker

Author Affiliations +

Proceedings Volume 11251, Label-free Biomedical Imaging and Sensing (LBIS) 2020; 112511Q (2020) https://doi.org/10.1117/12.2543625
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

We report the full-field imaging of the mechanical deformations accompanying the action potential in primary cortical neurons using ultrafast quantitative phase imaging (QPI) with a temporal resolution of 0.1 ms and a membrane displacement sensitivity of <0.2 nm per pixel. The average displacements were ~0.7 nm on cell somas and ~0.5 nm on neurites. Finite element modeling based on the 3D shape extracted from confocal imaging and on scaling of the surface tension with trans-membrane voltage yielded the deformation map during action potential, which matched the features of the experimental results, including the displacement amplitude, time course, and spatial distribution.

Conference Presentation

Citation Download Citation

Tong Ling, Kevin C. Boyle, Valentina Zuckerman, Thomas Flores, and Daniel V. Palanker "Full-field interferometric imaging of neuron deformation during action potential (Conference Presentation)", Proc. SPIE 11251, Label-free Biomedical Imaging and Sensing (LBIS) 2020, 112511Q (10 March 2020); https://doi.org/10.1117/12.2543625

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available