Development of collinear transmission plasmonic biosensor for detection of HIV-1

Saturnin Ombinda-Lemboumba; Sello Manoto; Charles Maphanga; Rudzani Malabi; Patience Mthunzi-Kufa

doi:10.1117/12.2546496

21 February 2020 Development of collinear transmission plasmonic biosensor for detection of HIV-1

Saturnin Ombinda-Lemboumba, Sello Manoto, Charles Maphanga, Rudzani Malabi, Patience Mthunzi-Kufa

Proceedings Volume 11257, Plasmonics in Biology and Medicine XVII; 112570L (2020) https://doi.org/10.1117/12.2546496
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Surface Plasmon Resonance (SPR) which is widely used to study interactions between different types of biomolecules, has emerged as a technique of choice for rapid and quantitative analyses. However, there are still some challenges on the use of the classical SPR optical configuration. The prism-based configuration setup requires precise alignment of light onto the sample surface and the oblique reflection angle plane yield optical aberration. In this work we have built, characterized and optimized a simple collinear transmission geometry plasmonic system for the detection of HIV-1. Here, a continuous wave laser at 785 nm with power output of 300 mW was used as light source and a 40X objective lens coupled to a CCD camera was used to collect and detect the transmitted intensity change. Furthermore, a white light source was used to study the wavelength dependency of the sample. We present our findings which may be useful to develop biomedical devices for point-of-care diagnostics and healthcare applications.

Conference Presentation

Citation Download Citation

Saturnin Ombinda-Lemboumba, Sello Manoto, Charles Maphanga, Rudzani Malabi, and Patience Mthunzi-Kufa "Development of collinear transmission plasmonic biosensor for detection of HIV-1", Proc. SPIE 11257, Plasmonics in Biology and Medicine XVII, 112570L (21 February 2020); https://doi.org/10.1117/12.2546496

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