Stimulated Raman voltage imaging for quantitative mapping of membrane potential

Hyeon Jeong Lee; Ji-Xin Cheng

doi:10.1117/12.2575378

10 October 2020 Stimulated Raman voltage imaging for quantitative mapping of membrane potential

Hyeon Jeong Lee, Ji-Xin Cheng

Proceedings Volume 11553, Optics in Health Care and Biomedical Optics X; 115531F (2020) https://doi.org/10.1117/12.2575378
Event: SPIE/COS Photonics Asia, 2020, Online Only

Abstract

Voltage imaging has become an emerging technique to record membrane potential change in living cells. Yet, compared to electrophysiology, microscopy approaches are still limited to relative membrane voltage changes, lacking important information conveyed by absolute membrane voltage. This talk will cover a spectroscopy approach to tackle this challenge. A spectroscopic signature of membrane potential was identified through stimulated Raman scattering (SRS) imaging, which enabled label-free, sub-cellular voltage imaging of mammalian neurons. We employed pre-resonance electronic absorption to enhance SRS imaging sensitivity and specificity. microbial rhodopsin voltage sensors, providing a quantitative approach to measure membrane potential. Quantitative voltage imaging by SRS has enabled mapping of absolute voltage in a neural network and has great potential in neurology and brain sciences.

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Hyeon Jeong Lee and Ji-Xin Cheng "Stimulated Raman voltage imaging for quantitative mapping of membrane potential", Proc. SPIE 11553, Optics in Health Care and Biomedical Optics X, 115531F (10 October 2020); https://doi.org/10.1117/12.2575378

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KEYWORDS

Raman spectroscopy

Neurons

Signal to noise ratio

Visualization

Action potentials

Brain

Calcium

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