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22 March 2021 Microscopic imaging of the effects of formaldehyde fixation on tissue autofluorescence
Shu-Jen Chiang, Po-Hang Tseng, Jing-Tang Lin, Chen-Yuan Dong
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Proceedings Volume 11648, Multiphoton Microscopy in the Biomedical Sciences XXI; 116481F (2021) https://doi.org/10.1117/12.2583984
Event: SPIE BiOS, 2021, Online Only
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Abstract
In this work, we investigated the effect of formaldehyde-induced autofluorescence using multiphoton and confocal microscopy. Porcine aorta, brain, kidney, and ventricle were processed, sliced, and immersed in 10% neutral buffered formalin at ambient temperature for different length of time. Our preliminary results show that the inducedautofluorescence in brain and ventricle tissue increases through time, whereas for aorta and kidney it remains nearly the same.
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Shu-Jen Chiang, Po-Hang Tseng, Jing-Tang Lin, and Chen-Yuan Dong "Microscopic imaging of the effects of formaldehyde fixation on tissue autofluorescence", Proc. SPIE 11648, Multiphoton Microscopy in the Biomedical Sciences XXI, 116481F (22 March 2021); https://doi.org/10.1117/12.2583984
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KEYWORDS
Tissues
Harmonic generation
Multiphoton microscopy
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