Time-resolved multi-spectral wide-field fluorescence lifetime imaging microscopy with a SPAD array detector

A. Ghezzi; A. Farina; A. Bassi; G. Valentini; I. Labanca; G. Acconcia; I. Rech; C. D'Andrea

doi:10.1117/12.2578683

5 March 2021 Time-resolved multi-spectral wide-field fluorescence lifetime imaging microscopy with a SPAD array detector

A. Ghezzi, A. Farina, A. Bassi, G. Valentini, I. Labanca, G. Acconcia, I. Rech, C. D'Andrea

Author Affiliations +

Proceedings Volume 11649, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVIII; 1164905 (2021) https://doi.org/10.1117/12.2578683
Event: SPIE BiOS, 2021, Online Only

Abstract

Multispectral Fluorescence Lifetime Imaging Microscopy (FLIM) is a fundamental tool to study multifold processes in biology and material science. The growing demand for acquisition time reduction requires the parallel acquisition of a multi-dimensional dataset and the exploitation of compressive sensing techniques. In this work we present a multispectral FLIM set-up based on wide-field structured illumination coupled with a spectrometer and a novel time-resolved parallel 18x1 SPAD array detector, working in a single pixel camera scheme. We show the system characterization and its imaging properties varying the compression ratio.

Conference Presentation

Citation Download Citation

A. Ghezzi, A. Farina, A. Bassi, G. Valentini, I. Labanca, G. Acconcia, I. Rech, and C. D'Andrea "Time-resolved multi-spectral wide-field fluorescence lifetime imaging microscopy with a SPAD array detector", Proc. SPIE 11649, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVIII, 1164905 (5 March 2021); https://doi.org/10.1117/12.2578683

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