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We present a super-resolving orientation microscopy method for single-particle imaging. In this method, the fluorescence signal from particles is imaged to obtain orientation and localization information simultaneously with higher throughput than photon counting methods. We demonstrate the technique by resolving nanocrystal quantum dot orientations within small clusters. The effects of orientation on coupling efficiency for energy transfer is investigated.
Duncan Ryan
"Camera-based single-particle orientation imaging of quantum dots", Proc. SPIE 11649, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVIII, 116490U (5 March 2021); https://doi.org/10.1117/12.2576811
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