Simultaneous recovery of phase and defocus from coherent through-focus intensity images

Jingshan Zhong; Emrah Bostan; Dihong Tian; Laura Waller

doi:10.1117/12.2575823

5 March 2021 Simultaneous recovery of phase and defocus from coherent through-focus intensity images

Jingshan Zhong, Emrah Bostan, Dihong Tian, Laura Waller

Proceedings Volume 11653, Quantitative Phase Imaging VII; 116530Z (2021) https://doi.org/10.1117/12.2575823
Event: SPIE BiOS, 2021, Online Only

Abstract

Phase retrieval methods have been proposed to recover phase from through-focus intensity images measured on a microscope. It requires measuring the defocus distances of intensity images, usually by a z-axis translation stage. However, it complicates the experiment, adds expense, and suffers from error in the z-stage measurement. In this paper, we propose an algorithm to recover phase from only through-focus intensity images without measuring extra parameters such as defocus, wavelength and pixel size. We formulate the phase retrieval as a least-square error optimization problem and propose an alternating update scheme to solve the nonconvex optimization problem. We validate the algorithm with simulation and experiment.

Conference Presentation

Citation Download Citation

Jingshan Zhong, Emrah Bostan, Dihong Tian, and Laura Waller "Simultaneous recovery of phase and defocus from coherent through-focus intensity images", Proc. SPIE 11653, Quantitative Phase Imaging VII, 116530Z (5 March 2021); https://doi.org/10.1117/12.2575823

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