High throughput droplet microfluidics for label-free analysis of individual cells

Yuval Atzitz; Itay Barnea; Natan Tzvi Shaked

doi:10.1117/12.2578171

5 March 2021 High throughput droplet microfluidics for label-free analysis of individual cells

Yuval Atzitz, Itay Barnea, Natan Tzvi Shaked

Proceedings Volume 11655, Label-free Biomedical Imaging and Sensing (LBIS) 2021; 116550V (2021) https://doi.org/10.1117/12.2578171
Event: SPIE BiOS, 2021, Online Only

Abstract

We propose a label-free technique that performs analysis of live individual cells during flow and then sort the cells based on the analysis results. The cells rapidly flow in a droplet microfluidics device, which prevents two cells in the field of view in once. By acquiring holograms of each cell during flow and achieving the cell optical path delay (OPD) profile, we extract different features that differentiate between the cells, based on which rapid sorting is performed. The device presents the possibility for both high-throughput label-free analysis of individual cells and their real-time sorting.

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Citation Download Citation

Yuval Atzitz, Itay Barnea, and Natan Tzvi Shaked "High throughput droplet microfluidics for label-free analysis of individual cells", Proc. SPIE 11655, Label-free Biomedical Imaging and Sensing (LBIS) 2021, 116550V (5 March 2021); https://doi.org/10.1117/12.2578171

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KEYWORDS

Microfluidics

Holograms

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