Stain-free 4D imaging of live sperm cells: the future of in-vitro fertilization

Natan Tzvi Shaked

doi:10.1117/12.2593193

20 June 2021 Stain-free 4D imaging of live sperm cells: the future of in-vitro fertilization

Natan Tzvi Shaked

Proceedings Volume 11786, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials V; 1178615 (2021) https://doi.org/10.1117/12.2593193
Event: SPIE Optical Metrology, 2021, Online Only

Abstract

One in six couples suffer from infertility problems. I will present our recent advances in the development of acquisition methods that enable high-resolution, fine-detail full reconstruction of the 3D movement and structure of individual human sperm cells swimming freely, in thousands of frames per second. We achieved the retrieval of the 3D refractive-index profile of the sperm head, revealing its fine internal organelles and orientation, and the detailed 4D localization of the thin and highly-dynamic sperm tail. We have also achieved virtual staining of individual sperm cells using deep learning. These stain-free imaging methods have great potential for both biological assays and clinical use of intact sperm cells during in-vitro fertilization procedures.

Conference Presentation

Citation Download Citation

Natan Tzvi Shaked "Stain-free 4D imaging of live sperm cells: the future of in-vitro fertilization", Proc. SPIE 11786, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials V, 1178615 (20 June 2021); https://doi.org/10.1117/12.2593193

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available