Presentation
3 August 2021 Using light to establish and measure stiffness gradients in three-dimensional engineered tissues
Author Affiliations +
Abstract
Studies of cell-extracellular matrix (ECM) interactions within fibrous systems such as collagen or fibrin are challenging, particularly if peri-cellular stiffness cannot be monitored. Here we present our light-based method for non-invasive patterning of molecular crosslinking combined with multi-axes optical tweezers active microrheology to map ECM stiffness landscapes. This method allows us to generate prescribed stiffness gradients and associated anisotropies, which model stiffness of the natural peri-cellular ECM. Patterned crosslinking induces strain hardening and measured stiffness gradients are in agreement with predicted strain fields. Migratory cells respond to these gradients as assessed by change in F-actin distribution and morphological properties.
Conference Presentation
© (2021) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Alicja Jagiello, Qingda Hu, Ulysses Castillo, and Elliot Botvinick "Using light to establish and measure stiffness gradients in three-dimensional engineered tissues", Proc. SPIE 11798, Optical Trapping and Optical Micromanipulation XVIII, 117980A (3 August 2021); https://doi.org/10.1117/12.2595590
Advertisement
Advertisement
KEYWORDS
Tissues

Anisotropy

3D modeling

Collagen

Optical fibers

Optical lithography

Optical tweezers

RELATED CONTENT


Back to Top