Polarized structured illumination microscopy

Peng Xi; Karl Zhanghao; Meiqi Li

doi:10.1117/12.2615382

27 October 2021 Polarized structured illumination microscopy

Peng Xi, Karl Zhanghao, Meiqi Li

Proceedings Volume 11925, Biomedical Imaging and Sensing Conference 2021; 1192502 (2021) https://doi.org/10.1117/12.2615382
Event: SPIE Technologies and Applications of Structured Light, 2021, Online, Japan

Abstract

Fluorescence polarization microscopy plays a vital role in studying molecular structures and dynamics of biocomplexes by imaging both the intensity and orientation of fluorescent dipoles. Super-resolution (SR) microscopy provides the ultrastructure of subcellular organelles, yet conventional SR lacks the dipole orientation information. We developed polarized structured illumination microscopy (pSIM) to achieve fluorescence super-resolution polarization microscopy.

Citation Download Citation

Peng Xi, Karl Zhanghao, and Meiqi Li "Polarized structured illumination microscopy", Proc. SPIE 11925, Biomedical Imaging and Sensing Conference 2021, 1192502 (27 October 2021); https://doi.org/10.1117/12.2615382

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