Whole-brain MR-registered cryo-imaging of a porcine-human glioma model to compare contrast agent biodistributions

B. K. Byrd; R. B. Duke; X. Fan; D. J. Wirth; P. J. Hoopes; W. R. Warner; R. R. Strawbridge; L. T. Evans; S. C. Davis; K. D. Paulsen

doi:10.1117/12.2608252

4 March 2022 Whole-brain MR-registered cryo-imaging of a porcine-human glioma model to compare contrast agent biodistributions

B. K. Byrd, R. B. Duke, X. Fan, D. J. Wirth, P. J. Hoopes, W. R. Warner, R. R. Strawbridge, L. T. Evans, S. C. Davis, K. D. Paulsen

Author Affiliations +

Proceedings Volume 11943, Molecular-Guided Surgery: Molecules, Devices, and Applications VIII; 1194303 (2022) https://doi.org/10.1117/12.2608252
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

As rapidly accelerating technology, fluorescence guided surgery (FGS) has the potential to place molecular information directly into the surgeon’s field of view by imaging administered fluorescent contrast agents in real time, circumnavigating pre-operative MR registration challenges with brain deformation. The most successful implementation of FGS is 5-ALAPpIX guided glioma resection which has been linked to improved patient outcomes. While FGS may offer direct in-field guidance, fluorescent contrast agent distributions are not as familiar to the surgical community as Gd-MRI uptake, and may provide discordant information from previous Gd-MRI guidance. Thus, a method to assess and validate consistency between fluorescence-labeled tumor regions and Gd-enhanced tumor regions could aid in understanding the correlation between optical agent fluorescence and Gd-enhancement. Herein, we present an approach for comparing whole-brain fluorescence biodistributions with Gd-enhancement patterns on a voxel-by-voxel basis using co-registered fluorescent cryo-volumes and Gd-MRI volumes. In this initial study, a porcine-human glioma xenograft model was administered 5-ALA-PpIX, imaged with MRI, and euthanized 22 hours following 5-ALA administration. Following euthanization, the extracted brain was imaged with the cryo-macrotome system. After image processing steps and non-rigid, point-based registration, the fluorescence cryo-volume and Gd-MRI volume were compared for similarity metrics including: image similarity, tumor shape similarity, and classification similarity. This study serves as a proof-of-principle in validating our screening approach for quantitatively comparing 3D biodistributions between optical agents and Gd-based agents.

Conference Presentation

Citation Download Citation

B. K. Byrd, R. B. Duke, X. Fan, D. J. Wirth, P. J. Hoopes, W. R. Warner, R. R. Strawbridge, L. T. Evans, S. C. Davis, and K. D. Paulsen "Whole-brain MR-registered cryo-imaging of a porcine-human glioma model to compare contrast agent biodistributions", Proc. SPIE 11943, Molecular-Guided Surgery: Molecules, Devices, and Applications VIII, 1194303 (4 March 2022); https://doi.org/10.1117/12.2608252

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available