LED-based light source for ultraviolet-visible absorption spectrophotometry

Takahiro Ando; Sakuichiro Adachi; Shoko Kawakami; Yuya Matsuoka; Yasuhiro Keta; Shohei Arita; Eiichiro Takada

doi:10.1117/12.2607165

7 March 2022 LED-based light source for ultraviolet-visible absorption spectrophotometry

Takahiro Ando, Sakuichiro Adachi, Shoko Kawakami, Yuya Matsuoka, Yasuhiro Keta, Shohei Arita, Eiichiro Takada

Proceedings Volume 11949, Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XX; 1194909 (2022) https://doi.org/10.1117/12.2607165
Event: SPIE BiOS, 2022, San Francisco, California, United States

Conference Poster

Abstract

Light-emitting diodes (LEDs) are widely used lighting applications that have several advantages over conventional lamp light sources, including higher mechanical robustness, faster response time, less energy consumption, and longer lifetime. In this study, we investigated the feasibility of LED-based absorption spectrophotometry. To implement an ultraviolet-visible (UV-VIS) absorption spectrophotometer, a light source was prototyped consisting of two types of LEDs: a UV LED (340 nm) and a broadband white LED (about 380-800 nm). A dichroic filter was used to combine the light of the two types of LEDs. Compared with conventional lamp light sources, the area of LED light emission is quite small and the emitting properties are not uniform. Therefore, to make the irradiance areas of two LEDs uniform, we applied an optical diffuser to the ultraviolet LED to widen the light-emitting area and broadly cover the irradiance areas from a white LED. This optical alignment enabled a configuration to withstand the mounting tolerance of approximately ±0.3 mm of LEDs on substrates. Another issue is that temperature must be stabilized because electrical and optical properties of LEDs strongly depend on temperature. Thus, we utilized a Peltier controller to precisely control temperature control of an LED-mounted substrate (less than ±0.01°C) and to achieve the target value of temporal drift within ±0.05% per hour of light intensity in photometric signals. The obtained results suggest that our developed LEDbased light source should be useful for a UV-VIS absorption spectrophotometer.

Conference Presentation

Citation Download Citation

Takahiro Ando, Sakuichiro Adachi, Shoko Kawakami, Yuya Matsuoka, Yasuhiro Keta, Shohei Arita, and Eiichiro Takada "LED-based light source for ultraviolet-visible absorption spectrophotometry", Proc. SPIE 11949, Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XX, 1194909 (7 March 2022); https://doi.org/10.1117/12.2607165

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available