Influence of annealing temperature on the cytotoxicity upconversion nanoparticles on different cell lines

R. A. Verkhovskii; R. A. Anisimov; M. V. Lomova; D. K. Tuchina; E. N. Lazareva; A. A. Doronkina; A. M. Mylnikov; N. A. Navolokin; V. I. Kochubey; I. Yu. Yanina

doi:10.1117/12.2621543

19 May 2022 Influence of annealing temperature on the cytotoxicity upconversion nanoparticles on different cell lines

R. A. Verkhovskii, R. A. Anisimov, M. V. Lomova, D. K. Tuchina, E. N. Lazareva, A. A. Doronkina, A. M. Mylnikov, N. A. Navolokin, V. I. Kochubey, I. Yu. Yanina

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Proceedings Volume 12147, Tissue Optics and Photonics II; 121470H (2022) https://doi.org/10.1117/12.2621543
Event: SPIE Photonics Europe, 2022, Strasbourg, France

Abstract

Carriers based on upconversion nanoparticles (UCNPs) and cyanine dye are suitable for theranostic application in oncology, although care must be taken for selection of the surface coating material, UCNP surface charge, size, and dosage of the material. Investigation of influence of annealing temperature of particles on the upconversion luminescence properties and cytotoxic effect are relevant. The present work demonstrates the assessment of cytotoxicity UCNPs unannealed and annealed at 550 oC on different normal and cancer murine cell lines in vitro. The cell viability is scored for cytotoxic effects of UCNPs at dark conditions. UCNPs provide a dose-dependent and time-dependent cytotoxic effect on all studied cell lines which was most pronounced for the Raw264.7 cell line. It is probably caused by the high phagocytic activity of macrophages. The statistically significant differences in cell viability after 24, 48 and 72 h of incubation of cells with particles were observed just for the macrophage cell line. It is also worth noting that annealed particles are less toxic than unannealed ones.

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R. A. Verkhovskii, R. A. Anisimov, M. V. Lomova, D. K. Tuchina, E. N. Lazareva, A. A. Doronkina, A. M. Mylnikov, N. A. Navolokin, V. I. Kochubey, and I. Yu. Yanina "Influence of annealing temperature on the cytotoxicity upconversion nanoparticles on different cell lines", Proc. SPIE 12147, Tissue Optics and Photonics II, 121470H (19 May 2022); https://doi.org/10.1117/12.2621543

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