Digital holography-based phase postprocessing and cell three-dimensional morphology analysis system

Zhuoshi Li; Qian Shen; Qian Chen; Chao Zuo

doi:10.1117/12.2642409

19 December 2022 Digital holography-based phase postprocessing and cell three-dimensional morphology analysis system

Zhuoshi Li, Qian Shen, Qian Chen, Chao Zuo

Proceedings Volume 12318, Holography, Diffractive Optics, and Applications XII; 1231824 (2022) https://doi.org/10.1117/12.2642409
Event: SPIE/COS Photonics Asia, 2022, Online Only

Conference Poster

Abstract

Digital holography microscopy (DHM), as a non-phototoxic non-destructive imaging technique, encodes a complex optical field into intensity modulations by the interference of scattered sample wave and reference wave and performs quantitative analysis of wave-matter interactions by decoding phase delay from a hologram. In this work, we propose a complete high-precision high-robust DHM-based quantitative phase microanalysis scheme (DHM-QPMS) for living cells. The whole scheme provides algorithmic support for 3D morphological information on cell area, circumference, irregularity, and volume. We quantitatively validated this scheme for the human cervical cancer (HeLa) cells under a ×20/0.45NA objective lens. Though the used sample is adherent and bizarre, the system can still achieve accurate target identification and cellular 3D feature extraction. Moreover, we believe that DHM-QPMS will be an important research tool for biological researchers.

Citation Download Citation

Zhuoshi Li, Qian Shen, Qian Chen, and Chao Zuo "Digital holography-based phase postprocessing and cell three-dimensional morphology analysis system", Proc. SPIE 12318, Holography, Diffractive Optics, and Applications XII, 1231824 (19 December 2022); https://doi.org/10.1117/12.2642409

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