Label-free identification of Alzheimer’s disease plaques using multiple co-registered nonlinear optical biomarkers

Kayvan F. Tehrani; Jaena Park; Carlos Renteria; Stephen A. Boppart

doi:10.1117/12.2648013

17 March 2023 Label-free identification of Alzheimer’s disease plaques using multiple co-registered nonlinear optical biomarkers

Kayvan F. Tehrani, Jaena Park, Carlos Renteria, Stephen A. Boppart

Author Affiliations +

Proceedings Volume 12364, Clinical and Translational Neurophotonics 2023; 1236405 (2023) https://doi.org/10.1117/12.2648013
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

This research presents our discovery of two new nonlinear optical biomarkers of Alzheimer’s disease (AD), namely 3-photon autofluorescence (3PAF), and Third Harmonic Generation (THG). A hallmark of AD is the aggregation of the Amyloid-Beta (Aβ) protein and Tau protein. Identification of these plaques and analysis of the surrounding cells and tissue is most often done using immunohistochemistry, often with inconsistent results. Using label-free nonlinear optical microscopy, new optical biomarkers were found for identifying a plaque. We present longitudinal imaging of AD progression in mice ranging from 8 to 52 weeks in age, in the hippocampal and cortical regions.

Conference Presentation

Citation Download Citation

Kayvan F. Tehrani, Jaena Park, Carlos Renteria, and Stephen A. Boppart "Label-free identification of Alzheimer’s disease plaques using multiple co-registered nonlinear optical biomarkers", Proc. SPIE 12364, Clinical and Translational Neurophotonics 2023, 1236405 (17 March 2023); https://doi.org/10.1117/12.2648013

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