Stabilized two-photon intravital imaging using dexmedetomidine

Youngkyu Kim; Minju Cho; Bjorn Paulson; Sung-Hoon Kim; Jun Ki Kim

doi:10.1117/12.2649582

6 March 2023 Stabilized two-photon intravital imaging using dexmedetomidine

Youngkyu Kim, Minju Cho, Bjorn Paulson, Sung-Hoon Kim, Jun Ki Kim

Proceedings Volume 12368, Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XXI; 123680N (2023) https://doi.org/10.1117/12.2649582
Event: SPIE BiOS, 2023, San Francisco, California, United States

Conference Poster

Abstract

Two-photon microscopy provides subcellular-resolution imaging deep into animal tissues, and is frequently used for the study of biological and disease processes. However, its applicability in vivo is complicated by physiological movement. We demonstrate treatment with dexmedetomidine (DEX), an already FDA-approved alpha-2 adrenergic receptor agonist, to reduce tissue oscillation frequency and amplitude in the livers of anesthetized mice. Fluorescence intensity and focal quality fluctuations were found to improve for 30 minutes after administration, indicating that dexmedetomidine may be used to improve imaging quality in two-photon intravital microscopy studies. These results will likely generalize to other imaging modalities, target tissues, and animal models.

Citation Download Citation

Youngkyu Kim, Minju Cho, Bjorn Paulson, Sung-Hoon Kim, and Jun Ki Kim "Stabilized two-photon intravital imaging using dexmedetomidine", Proc. SPIE 12368, Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XXI, 123680N (6 March 2023); https://doi.org/10.1117/12.2649582

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