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Frequency-domain near-infrared spectroscopy (FD-NIRS) can measure absolute tissue optical properties for functional brain imaging. Multiple source-detector separations (SDS), and multiple modulation frequencies can be used for FD-NIRS application in homogeneous or multi-layer tissue models. A multiple-frequency approach is advantageous in building more compact systems with a single SDS. In this work, we compare the accuracy of estimating absolute optical properties of a multi-layered tissue model using multi-distance and multi-frequency approaches. We demonstrate that the multiple-frequency approach is comparable in accuracy to the multiple-distance approach and can be confidently implemented for brain imaging applications.
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Ola Abdalsalam, Nicholas A. Ross, Guy A. Perkins, Robin Dale, Hamid Dehghani, Thomas D. O'Sullivan, Silvina L. Ferradal, "Comparison of multi-distance and multi-frequency methods in frequency-domain near-infrared spectroscopy," Proc. SPIE 12376, Optical Tomography and Spectroscopy of Tissue XV, 1237607 (7 March 2023); https://doi.org/10.1117/12.2650970