Caroline E. Filan,1 Seleipiri Charles,1,2 Paloma Casteleiro Costa,1 Brian Cheng,1,2 Hang Lu,1 Francisco E. Robleshttps://orcid.org/0000-0003-4413-97791,2
1Georgia Institute of Technology (United States) 2Emory Univ. (United States)
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Quantitative oblique back-illumination microscopy (qOBM) is a label-free imaging technique that enables tomographic phase imaging of thick scattering samples with epi-illumination. Here, we apply qOBM to image three-dimensional brain organoid cell cultures of tuberous sclerosis complex (TSC) disease. We identify quantitative differences that occur between the TSC organoids and a control cell line, and discuss the implications of these differences on our understanding the development of TSC organoid cultures. These differences include disruptions in the tubular processes in the organoid, a higher degree of folding and non-spherical cell growth, and differences in the proliferating cell structures between the two groups.
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Caroline E. Filan, Seleipiri Charles, Paloma Casteleiro Costa, Brian Cheng, Hang Lu, Francisco E. Robles, "Tracking early tuberous sclerosis complex diseased organoid development with quantitative oblique back-illumination microscopy," Proc. SPIE 12383, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI, 123830E (15 March 2023); https://doi.org/10.1117/12.2650755