Speckle flow structured illumination microscopy for dynamic super-resolution imaging

Ruiming Cao; Fanglin Linda Liu; Li-Hao Yeh; Guanghan Meng; Laura Waller

doi:10.1117/12.2648072

16 March 2023 Speckle flow structured illumination microscopy for dynamic super-resolution imaging

Ruiming Cao, Fanglin Linda Liu, Li-Hao Yeh, Guanghan Meng, Laura Waller

Proceedings Volume 12390, High-Speed Biomedical Imaging and Spectroscopy VIII; 1239002 (2023) https://doi.org/10.1117/12.2648072
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

We propose a new method, Speckle Flow SIM, to super-resolve every frame of a dynamic scene from a sequence of diffraction-limited images at different acquisition timepoints. Speckle Flow SIM uses a fixed speckle-structured illumination to encode the super-resolved information into measurements and a neural space-time model to exploit the temporal redundancy for the dynamic scene reconstruction. We built a simple, inexpensive setup for Speckle Flow SIM and experimentally demonstrated 1.88× super-resolution for a dynamic scene.

Conference Presentation

Citation Download Citation

Ruiming Cao, Fanglin Linda Liu, Li-Hao Yeh, Guanghan Meng, and Laura Waller "Speckle flow structured illumination microscopy for dynamic super-resolution imaging", Proc. SPIE 12390, High-Speed Biomedical Imaging and Spectroscopy VIII, 1239002 (16 March 2023); https://doi.org/10.1117/12.2648072

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