Silica nanoparticles coating in silicon nitride photonic label-free biosensing of S100 calcium-binding protein A6

Suruk Udomsom; Phornsawat Baipaywad; Pathinan Paengnakorn; Ukrit Mankong; Nipon Theera-Umpon; Toshimasa Umezawa; Atsushi Matsumoto; Atsushi Kanno; Naokatsu Yamamoto; Kouichi Akahane; Manuel Mendez-Astudillo; Nithi Atthi; Wittawat Yamwong; Nipapan Klunngien; Rattanawan Meananeatra; Kitti Intuyod; Somchai Pinlaor; Tetsuya Kawanishi; Kazuo Imai

doi:10.1117/12.2648777

17 March 2023 Silica nanoparticles coating in silicon nitride photonic label-free biosensing of S100 calcium-binding protein A6

Suruk Udomsom, Phornsawat Baipaywad, Pathinan Paengnakorn, Ukrit Mankong, Nipon Theera-Umpon, Toshimasa Umezawa, Atsushi Matsumoto, Atsushi Kanno, Naokatsu Yamamoto, Kouichi Akahane, Manuel Mendez-Astudillo, Nithi Atthi, Wittawat Yamwong, Nipapan Klunngien, Rattanawan Meananeatra, Kitti Intuyod, Somchai Pinlaor, Tetsuya Kawanishi, Kazuo Imai

Author Affiliations +

Proceedings Volume 12395, Colloidal Nanoparticles for Biomedical Applications XVIII; 1239502 (2023) https://doi.org/10.1117/12.2648777
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Efficient functionalization of the silicon nitride waveguide with bioreceptors, e.g. antibodies, is key to increase antigen binding activity in photonic sensors based on silicon nitride (SiN) waveguides. A bioreceptor coating technique using silica nanoparticles (NPs) to enhance the density of functionalized antibodies, by increasing the surface areas for biomolecule binding, is proposed. The detection of S100 Calcium-Binding Protein A6 (S100A6), a proposed cholangiocarcinoma marker, has been demonstrated using the SiN resonator sensor with 400nm thick waveguide, fabricated by low-cost 500 nm technology. The NPs were synthesized by silica condensation. Antibodies were attached to the NPs by 1-ethyl-3-(3- dimethyl aminopropyl)–carbodiimide (EDC)/ N-hydroxysuccinimide (NHS)-crosslinking. Then the NPs were coated on SiN sensor by N-terminal to N-terminal crosslinkers. It was found that the application of silica NPs coating showed increased sensor sensitivity at approximately 8.8 pm/(ng/ml) in optical resonant wavelength shift compared to 0.36 pm/(ng/ml) by our previous antibody coating technique using (3-Aminopropyl) triethoxysilane (APTES) silanization with EDC/NHS protein crosslink.

Conference Presentation

Citation Download Citation

Suruk Udomsom, Phornsawat Baipaywad, Pathinan Paengnakorn, Ukrit Mankong, Nipon Theera-Umpon, Toshimasa Umezawa, Atsushi Matsumoto, Atsushi Kanno, Naokatsu Yamamoto, Kouichi Akahane, Manuel Mendez-Astudillo, Nithi Atthi, Wittawat Yamwong, Nipapan Klunngien, Rattanawan Meananeatra, Kitti Intuyod, Somchai Pinlaor, Tetsuya Kawanishi, and Kazuo Imai "Silica nanoparticles coating in silicon nitride photonic label-free biosensing of S100 calcium-binding protein A6", Proc. SPIE 12395, Colloidal Nanoparticles for Biomedical Applications XVIII, 1239502 (17 March 2023); https://doi.org/10.1117/12.2648777

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available