Protein immobilization in accumulated pyro-electrohydrodynamic droplets for highly sensitive detection of astronaut health biomarkers

Concetta Di Natale; Sara Coppola; Veronica Vespini; Volodymyr Tkachenko; Giuseppina Luciani; Giuseppe Vitiello; Francesca Ferranti; Silvia Mari; Pier Luca Maffettone; Simonetta Grilli

doi:10.1117/12.2675813

11 August 2023 Protein immobilization in accumulated pyro-electrohydrodynamic droplets for highly sensitive detection of astronaut health biomarkers

Concetta Di Natale, Sara Coppola, Veronica Vespini, Volodymyr Tkachenko, Giuseppina Luciani, Giuseppe Vitiello, Francesca Ferranti, Silvia Mari, Pier Luca Maffettone, Simonetta Grilli

Author Affiliations +

Proceedings Volume 12622, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials VI; 126220Z (2023) https://doi.org/10.1117/12.2675813
Event: SPIE Optical Metrology, 2023, Munich, Germany

Abstract

The presence of microgravity and ionizing radiation during spaceflight missions causes excessive Reactive Oxygen Species (ROS) production that contributes to oxidative cellular stress and multifunctional damage in astronauts. This knowledge has underlined the importance of frequent monitoring of astronaut’s health to have early diagnoses. In this scenario, the biosensor diagnostic devices could offer the necessary analytical performance to study pathological astronaut conditions. Herein, we propose an innovative biosensor for detecting highly diluted biomarkers at picogram level by using the pyro-electrohydrodynamic jet (p-jet) system. The detection limit of the system was confirmed using a model protein as the Bovine Serum Albumin (BSA) by optimizing its deposition on different functionalized glass substrates through different chemical reactions starting with a manual procedure. Based on these results, the epoxy glass activated surface was chosen as the best slide for p-jet experiments. The characterization of the processes was performed through different spectroscopic techniques such as infrared-spectroscopy (IR) or confocal fluorescence. In the context of long-term human missions, our revolutionary approach could be extremely useful to monitor the astronaut health.

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Concetta Di Natale, Sara Coppola, Veronica Vespini, Volodymyr Tkachenko, Giuseppina Luciani, Giuseppe Vitiello, Francesca Ferranti, Silvia Mari, Pier Luca Maffettone, and Simonetta Grilli "Protein immobilization in accumulated pyro-electrohydrodynamic droplets for highly sensitive detection of astronaut health biomarkers", Proc. SPIE 12622, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials VI, 126220Z (11 August 2023); https://doi.org/10.1117/12.2675813

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KEYWORDS

Proteins

Biosensors

Glasses

Fluorescence

Chemical reactions

Epoxies

Crystals

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