Dynamic speckle illumination wide-field fluorescence microscopy based on active light manipulation

Jun Yin; Yan Miao; Lingyao Yu

doi:10.1117/12.2687088

29 November 2023 Dynamic speckle illumination wide-field fluorescence microscopy based on active light manipulation

Jun Yin, Yan Miao, Lingyao Yu

Proceedings Volume 12767, Optoelectronic Imaging and Multimedia Technology X; 1276716 (2023) https://doi.org/10.1117/12.2687088
Event: SPIE/COS Photonics Asia, 2023, Beijing, China

Conference Poster

Abstract

In order to obtain the fine structure of biological tissues and living cells, microscopic imaging techniques are required to have tomographic capabilities. We propose a dynamic speckle fluorescence illumination microsystem based on active light manipulation, which uses the dynamically changing speckle pattern to illuminate the sample to be measured in full field, rotates the sample to be measured through linear laser tweezers, and obtains a three-dimensional fluorescence tomography image by extracting the fluorescence signal that changes sharply in the focal plane. The system changes the previous passive observation to active observation, and realizes the acquisition of three-dimensional structural chromatographic images with high temporal and spatial resolution without affecting the nature of the cells themselves and their environment, and the long-term dynamic quantitative monitoring of their life activity processes.

Citation Download Citation

Jun Yin, Yan Miao, and Lingyao Yu "Dynamic speckle illumination wide-field fluorescence microscopy based on active light manipulation", Proc. SPIE 12767, Optoelectronic Imaging and Multimedia Technology X, 1276716 (29 November 2023); https://doi.org/10.1117/12.2687088

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