Quantifying the extravasating distance of fluorescence imaging agent from blood vessels into surrounding tissues

Yao Chen; Sassan Hodge; Kimberley S. Samkoe

doi:10.1117/12.3002525

12 March 2024 Quantifying the extravasating distance of fluorescence imaging agent from blood vessels into surrounding tissues

Yao Chen, Sassan Hodge, Kimberley S. Samkoe

Author Affiliations +

Proceedings Volume 12825, Molecular-Guided Surgery: Molecules, Devices, and Applications X; 1282509 (2024) https://doi.org/10.1117/12.3002525
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

To assess the agent penetration in tissue, we developed two methodologies of image analysis for quantifying the extravasation distances of fluorescent imaging agents: Perpendicular Line Intensity Profiling (PLIP) and Iterative Radial Dilation Profiling (IRDP). Utilizing automated vessel identification and fluorescence microscopy, these methods provide a robust framework for analyzing agent distribution in tumor tissues. Our findings demonstrate that the IRDP method significantly mitigates noise, offering a smoother intensity decay profile compared to PLIP. This advantage suggests that IRDP could improve the reliability of assessing agent delivery and penetration, which are critical for providing effective imaging contrast in FGS. By comparing agent penetration profiles, we advocate for the broader adoption of the IRDP method in future quantitative fluorescence imaging analyses to enhance assessment accuracy and consistency.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Yao Chen, Sassan Hodge, and Kimberley S. Samkoe "Quantifying the extravasating distance of fluorescence imaging agent from blood vessels into surrounding tissues", Proc. SPIE 12825, Molecular-Guided Surgery: Molecules, Devices, and Applications X, 1282509 (12 March 2024); https://doi.org/10.1117/12.3002525

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