Quantification of viral particles using a photonic crystal biosensor

Masixole Yvonne Lugongolo; Saturnin Ombinda-Lemboumba; Charles Maphanga; Phumulani Michael Mcoyi; Ahmed El-Hussein; Patience Mthunzi-Kufa

doi:10.1117/12.3002395

13 March 2024 Quantification of viral particles using a photonic crystal biosensor

Masixole Yvonne Lugongolo, Saturnin Ombinda-Lemboumba, Charles Maphanga, Phumulani Michael Mcoyi, Ahmed El-Hussein, Patience Mthunzi-Kufa

Author Affiliations +

Proceedings Volume 12860, Plasmonics in Biology and Medicine XXI; 1286002 (2024) https://doi.org/10.1117/12.3002395
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

The quantification of human immunodeficiency virus at point of care remains a challenge in resource limited settings. The incorporation of nanotechnology and label free optical biosensing has unlocked promising opportunities in the development of diagnostic tools for infectious diseases. Optical biosensors offer a rapid and sensitive optical method for various biological materials such as cells, biomolecules, and viruses by monitoring the dielectric permittivity changes at the interface of a transducer substrate and the analyte. This work focuses on exploring photonic crystal biosensor efficiency and sensitivity for viral load measurement. Photonic crystal biosensors are a unique class of biosensors that allow for label free analysis as they can control and confine light propagation due to the photonic bandgap. Silane treated photonic crystal was functionalized with anti-HIV-gp120 antibody before the addition of various concentrations of HIV pseudovirus. The samples were analyzed on a custom build transmission spectroscopy that used white light as a light source. The results showed a red shift at different virus concentrations, which demonstrates that photonic crystal biosensors are sensitive enough to detect differences in virus concentrations. Therefore, photonic crystals have a potential in the development of photonic crystal-based biosensors for viral load detection.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Masixole Yvonne Lugongolo, Saturnin Ombinda-Lemboumba, Charles Maphanga, Phumulani Michael Mcoyi, Ahmed El-Hussein, and Patience Mthunzi-Kufa "Quantification of viral particles using a photonic crystal biosensor", Proc. SPIE 12860, Plasmonics in Biology and Medicine XXI, 1286002 (13 March 2024); https://doi.org/10.1117/12.3002395

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available