Determination of operating parameters of fungal growth signals analyzed by laser speckle contrast imaging

Ilya Balmages; Aigars Reinis; Svjatoslavs Kistkins; Janis Liepins; Maksym Pogorielov; Viktoriia Korniienko; Kateryna Diedkova; Dmitrijs Bliznuks; Alexey Lihachev; Ilze Lihacova

doi:10.1117/12.3016906

20 June 2024 Determination of operating parameters of fungal growth signals analyzed by laser speckle contrast imaging

Ilya Balmages, Aigars Reinis, Svjatoslavs Kistkins, Janis Liepins, Maksym Pogorielov, Viktoriia Korniienko, Kateryna Diedkova, Dmitrijs Bliznuks, Alexey Lihachev, Ilze Lihacova

Author Affiliations +

Proceedings Volume 13006, Biomedical Spectroscopy, Microscopy, and Imaging III; 1300618 (2024) https://doi.org/10.1117/12.3016906
Event: SPIE Photonics Europe, 2024, Strasbourg, France

Conference Poster

Abstract

Our previous studies have shown that laser speckle imaging with sensitive subpixel correlation analysis is able to detect bacterial growth activity and the pattern of colony growth. In the current study, we demonstrate the potential of this method to analyze fungal growth. We compare the characteristics of the signals obtained from bacteria and fungi. The obtained results will help to improve the parameters of the speckle image acquisition system and the signal processing algorithms useful for microorganism (both eukaryotic and prokaryotic) growth analyses and speeding up and facilitating microbiological diagnostics.

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Ilya Balmages, Aigars Reinis, Svjatoslavs Kistkins, Janis Liepins, Maksym Pogorielov, Viktoriia Korniienko, Kateryna Diedkova, Dmitrijs Bliznuks, Alexey Lihachev, and Ilze Lihacova "Determination of operating parameters of fungal growth signals analyzed by laser speckle contrast imaging", Proc. SPIE 13006, Biomedical Spectroscopy, Microscopy, and Imaging III, 1300618 (20 June 2024); https://doi.org/10.1117/12.3016906

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